Enabling the Twist Human Core Exome Kit on Miro CanvasWhole exome sequencing (WES) provides a unique opportunity to dive deeply into the coding regions of the genome. It plays an important role in generating data for research, and in some cases clinical applications. Miro Canvas also gives users flexibility to utilize the true “walk-away” automation, this application note describes results when using the Twist Bioscience Human Core Exome kit.
Key points include:
- Library preparation and hybridization capture using Twist Bioscience’s Human Core Exome Kit are automated on Miro Canvas
- These protocols have been developed using 50ng DNA input for library prep and 1500ng for hybridization capture
- Depth of coverage, quality scores, and other key metrics are comparable between manually prepared libraries and those run on Miro Canvas