App Note
Automating the Oxford Nanopore Ligation Sequencing Kit on Miro Canvas
Long read sequencing is particularly well suited for detection of large genomic mutations, coverage of long repeat regions that confound short read assemblies, and identification of signatures that can be lost due to PCR amplification.
Key points include:
- Library preparation using the 1D ligation kit from ONT is fully automated on Miro Canvas
- The protocol uses 25% reaction of volumes compared to manual library preparation using 1 μg high quality, high molecular weight input DNA
- High quality results are achieved, the N50 and other key metrics are comparable to manual
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