Automating Pacbio WGS Library Prep on the Miro CanvasLong read sequencing plays an important role in generating contiguous, high-quality genomes, for haplotype phasing, structural variant detection, and de novo assemblies. The application note describes results using the SMRTbell Express Template Kit 2.0 and SMRTbell Prep Kit 3.0 in protocols developed for Miro Canvas.
Key points include:
- WGS library preparation is fully automated on Miro Canvas using 1-5 μg high quality, high molecular weight input DNA
- Flexibility to choose an automated, fast bead-based size selection, or a more stringent gel-based size selection
- Miro Canvas total library quantities, peak sizes, and primary sequencing metrics are indistinguishable from manually prepared libraries